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Quartz tuning fork-based microviscometry for selecting high-quality sperm from semen

이만희 교수

충북대학교 물리학과

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mlee@cbnu.ac.kr

Quartz tuning fork-based microviscometry for selecting high-quality sperm from semen

Manhee Lee^*

Department of Physics, Research Institute for Nanoscale Science & Technology, Chungbuk National University, Cheongju 28644, Chungbuk, Korea

*mlee@cbnu.ac.kr

The viscometry of minute liquid amounts is crucial for biomedical diagnosis and engineering. However, interfacial effects complicate measurements and analysis, especially for nanoliter samples. Here, we present a quartz tuning fork-based microviscometry technique that determines the viscosity of single sessile drops at the nanoliter scale. We circumvent the interfacial effects by measuring the negative-valued shear elasticity, originating purely from the retarded fluidic response inside the drop. This method provides an exact formula for viscosity determination from the shear elasticity.

We applied this viscometry technique to create sperm sorting media with appropriate viscosity, enabling the selection of high-quality sperm in conjunction with microfluidic techniques. We show that highly motile sperm can be separated in a viscous medium, where the sperm actively diffuses throughout the medium depending on their motility. We separate motile sperm from semen and identify bovine blastocysts through in vitro fertilization of matured oocytes. Our quartz tuning fork-based microviscometry technique has potential applications in clinical diagnosis and treatment.

(a)	(b)

Figure 1. (a) Schematic representation of the quartz tuning fork-based micro-viscometer and (b) Trajectories of all objects including sperm and debris in each medium: M199 (PVP 0%), PVP 0.75%, and PVP 1.5%.

[1] D. G. Byeun†, D. Kim†, J. H. Park†, M. Lee* and J. K. Choi*, Analyst 149, 3078-3084 (2024).

[2] M. Lee et al., Biomedicines 9(10), 1439 (2021).

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